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Morphology of renal structures in kidney organoids generated with the original APEL/air-medium interface protocol. Representative immunofluorescence images of the maturing glomerular structures, stained for nephrin, and maturing tubular structures, stained for epithelial cadherin (ECAD) in healthy-donor iPSC lines HEL24.3 and HEL61.2, and the GRACILE iPSC line HEL124.2, images acquired with (A) widefield fluorescence microscopy, scale bar 500µm, and (B) the Opera Phenix spinning disk confocal <t>microscope,</t> scale bar 200µm.
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Morphology of renal structures in kidney organoids generated with the original APEL/air-medium interface protocol. Representative immunofluorescence images of the maturing glomerular structures, stained for nephrin, and maturing tubular structures, stained for epithelial cadherin (ECAD) in healthy-donor iPSC lines HEL24.3 and HEL61.2, and the GRACILE iPSC line HEL124.2, images acquired with (A) widefield fluorescence microscopy, scale bar 500µm, and (B) the Opera Phenix spinning disk confocal microscope, scale bar 200µm.

Journal: bioRxiv

Article Title: Parsing glomerular and tubular structure variability in high-throughput kidney organoid culture

doi: 10.1101/2025.05.05.652160

Figure Lengend Snippet: Morphology of renal structures in kidney organoids generated with the original APEL/air-medium interface protocol. Representative immunofluorescence images of the maturing glomerular structures, stained for nephrin, and maturing tubular structures, stained for epithelial cadherin (ECAD) in healthy-donor iPSC lines HEL24.3 and HEL61.2, and the GRACILE iPSC line HEL124.2, images acquired with (A) widefield fluorescence microscopy, scale bar 500µm, and (B) the Opera Phenix spinning disk confocal microscope, scale bar 200µm.

Article Snippet: Kidney organoids were also imaged using PerkinElmer Opera Phenix spinning disk confocal microscope.

Techniques: Generated, Immunofluorescence, Staining, Fluorescence, Microscopy